Fig. 3: Deletion of proteins with pDC and dDC-like localisations cause minor changes in axonemal structure.

A, B. Box and whisker plot of flagellum length in deletion mutants. Points represent the median; box and whiskers represent the quartile ranges and the 5th and 95th percentile. n indicates the number of cells. P-values were calculated using a Student’s t-test (two tailed), p values <0.05 are shown. Source data are provided in the Source Data file. C–E Thin-section electron microscopy of axoneme structure. The first column of each shows one representative axoneme cross-section in the flagellar pocket or in the free flagellum; the second column shows an averaged axoneme structure, in which axoneme cross-sections have had perspective deviation from circularity corrected (n indicates the number of axonemes used); the third column shows the result of ninefold rotational averaging and averaging across multiple axonemes; the fourth column shows a zoomed view of one microtubule doublet. C Analysis of the proximal flagellum for deletion mutants of proximal axoneme proteins. D Distal flagellum for deletion mutants of distal axoneme proteins. E Proximal and distal flagellum for double deletion mutant of the proximal and distal paralogous pair LmxM.29.0240 and LmxM.30.0090 in comparison to a pDC1 and dDC2 double deletion mutant. Circles in C–E highlight changes in electron density.