Fig. 5: ODAβ deletion limits ODAα incorporation in the proximal axoneme while ODAβ requires ODAα for axonemal incorporation.
From: Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency
A First column: micrographs of L. mexicana cell line expressing ODAα or ODAβ tagged with mNG at the C terminus. Second column: after deletion of both alleles of ODAα or ODAβ. Phase contrast (grey), DNA (Hoeschst 33342, magenta) and mNG (green) overlay and mNG fluorescence are shown. Cell lines where the protein fails to localise to the axoneme are outlined in orange, and cell lines where the localisation is axonemal but changed are outlined in blue. B Ultrastructure changes upon ODAα or ODAβdeletion. First and fifth columns: one representative axoneme cross-section in the flagellar pocket and in the free flagella. Second and sixth columns: an averaged axoneme structure. (n indicates the number of axonemes used). Third and seventh columns: an electron density difference map, resulting from subtraction of deletion mutant average axoneme image from the parental cell line. Yellow indicates a loss of electron density in the deletion mutant, magenta indicates gain.
