Table 1 Kinetic parameters and associated 95% confidence intervals of each 20S variant against Z-VLR-AMC
From: Structural basis for allosteric modulation of M. tuberculosis proteasome core particle
Variant | K0.5 (µM) | 95% C.I. K0.5 (µM) | n | 95% C.I. n | Vmax (µM s–1) | 95% C.I. Vmax (µM s–1) |
|---|---|---|---|---|---|---|
20SWT | 94.5 | 79.9 – 111.7 | 1.6 | 1.4 – 1.7 | 3.1 | 2.8 – 3.5 |
20SOG | 39.1 | 35.0 – 43.7 | 2.3 | 1.9 – 2.7 | 3.4 | 3.1 – 3.7 |
20SαK52F/βV53Q | 95.3 | 68.9 – 131.8 | 1.8 | 1.4 – 2.1 | 8.1 | 6.1 – 10.1 |
20SβV53Q | 87.8 | 65.8 – 117.0 | 1.7 | 1.3 – 2.0 | 6.0 | 4.8 – 7.1 |
20SαK52F | 59.3 | 50.5 – 69.8 | 1.9 | 1.6 – 2.2 | 4.2 | 3.7 – 4.7 |
20SαS17F | 57.5 | 43.6 – 75.9 | 1.8 | 1.3 – 2.2 | 3.2 | 26 – 3.9 |
20SβY35F | 68.5 | 58.5 – 80.2 | 1.9 | 1.6 – 2.2 | 1.4 | 1.3 – 1.6 |
20SαQ98K | 73.8 | 59.0 – 88.5 | 2.2 | 1.5 – 2.9 | 1.0 | 0.9 – 1.2 |
20SβA100S | – | – | – | – | – | – |
20SβA92-94G | – | – | – | – | – | – |
