Fig. 2: Oligomerization states of 3 Sm subcomplexes from S. cerevisiae and their interactions. | Nature Communications

Fig. 2: Oligomerization states of 3 Sm subcomplexes from S. cerevisiae and their interactions.

From: A unique mechanism of snRNP core assembly

Fig. 2: Oligomerization states of 3 Sm subcomplexes from S. cerevisiae and their interactions.

Interactions among Sm subcomplexes were tested by a pull-down assay. In each reaction, only one Sm subcomplex, D1/D2 (a) or F/E/G (b), was tagged with His-TEV (HT-), while the other subcomplex(es) had the HT-tag removed. The indicated Sm subcomplexes were incubated and then pulled down using Ni-beads. M markers. The oligomerization states of D3/B (c), D1/D2 (d), and F/E/G (e) and the interaction between D1/D2 and F/E/G (f) were tested using GFC (left panels). The eluted fractions (indicated by red bars) were then analyzed by SDS-PAGE/CBB staining (right panels). For each set, a representative result from two independent experiments is shown. g Summary of the oligomerization states and interactions of the three Sm subcomplexes.

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