Fig. 2: High-throughput DNA sequencing fingerprints of naïve DP-DEL and control selections. | Nature Communications

Fig. 2: High-throughput DNA sequencing fingerprints of naïve DP-DEL and control selections.

From: Flexibility-tuning of dual-display DNA-encoded chemical libraries facilitates cyclic peptide ligand discovery

Fig. 2

A High-throughput DNA sequencing (HTDS) results of unselected DP-DEL represented through a 3D plot displaying the distribution of the most prominent BBs 1-3 where each axis represents a BB position and each dot is a library member carrying a combination of said BBs, while the heat bar represents sequence counts. No cut-off was applied for unselected library. B, C Positive control concatenated triplicate HTDS against CAIX and NSP14 respectively. Spiked-in sulfonamide BB1-175 and BB3-176 showed preferential enrichment as binders for CAIX. Spiked-in S-adenosylhomocysteine BB1-119 and BB3-137 as a combination showed preferential enrichment as binders against NSP14. A cut-off of 20 counts was set for CAIX and NSP14. Selections were performed in triplicate and concatenated into a single dataset for visual display (for the selection results with the individual DP-DEL versions see Supplementary Information Fig. S28).

Back to article page