Fig. 5: Naïve T cells in aged patients have a unique phenotype compared to young patients before and after ICI treatment. | Nature Communications

Fig. 5: Naïve T cells in aged patients have a unique phenotype compared to young patients before and after ICI treatment.

From: Age-related divergence of circulating immune responses in patients with solid tumors treated with immune checkpoint inhibitors

Fig. 5: Naïve T cells in aged patients have a unique phenotype compared to young patients before and after ICI treatment.The alternative text for this image may have been generated using AI.

Heatmaps were generated for the average scaled mean metal intensity (MMI) between aged (n = 49) and young patients (n = 42) for 12 markers of interest related to T cell memory, effector/proliferation function, and exhaustion for 15 unique immune clusters at baseline and on treatment. Scaled MMIs were calculated for each individual marker within each immune cluster as defined from the annotation clustering from Fig. 3A. Scaling was performed for visualization purposes to highlight the most divergent markers by age group, and formal statistical comparisons were performed with a two-sided Wilcoxon rank-sum test on non-scaled MMIs without adjustment for multiple comparisons, with statistically significant comparisons (P < 0.05) indicated on the heatmaps with a star in the box of the more highly expressed marker along with absolute MMIs on box and whisker plots showing the median, interquartile range (IQR), minimum/maximum values, and additional marking of outliers. Results for ThN (A, B) and TcN (C, D) are presented. Analysis of PD-1 (PD1) expression in post-treatment samples was not included due to use of a competitive antibody as described in the methods. represents naïve immune clusters as labeled. Source data are provided as a Source Data file.

Back to article page