Fig. 4: PDZD8 and FKBP8 tether the ER and mitochondria cooperatively.
From: Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering
a Representative electron micrographs of Pdzd8f/f::CreERT2 MEFs infected with lentivirus carrying shControl or shFKBP8, and treated with or without 0.5 µM 4-OHT. MERCS (yellow arrowheads) were more frequently observed in the Control cells than in Pdzd8 cKO, Fkbp8 KD, and Pdzd8 cKO + Fkbp8 KD cells. Scale bars: 200 nm. b Quantification of the MERCS length normalized by the mitochondrial circumference. The data are presented as individual points on box plots, with the center indicating the median, and the 25th and 75th percentiles represented by the box. Whiskers extend to the minimum and maximum values. n = 33, 29, 39, 34 cells from two independent experiments for the control, Pdzd8 cKO, Fkbp8 KD, and Pdzd8 cKO + Fkbp8 KD cells, respectively. Statistical analysis was performed using one-way ANOVA and Fisher’s LSD test. ****p < 0.0001, ***p = 0.0003. c The interaction plot corresponding to b. Dots show the mean of each condition. d The results of the two-way ANOVA test. The low (<0.01) variation of the interaction shows that PDZD8 and FKBP8 cooperatively affect the areas of MERCS. Source data are provided as a Source Data file.
