Fig. 8: Volume EM analysis revealed that PDZD8 promotes mitochondrial complexity by inhibiting FKBP8.
From: Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering
a Schematic of mitochondrial morphology analysis by the volume EM. Serial electron micrographs were obtained by imaging the serial sections with an FE–SEM. After cropping the volume, the mitochondria in the volume were semi-automatically extracted with an AI-assisted pipeline. b Formula of calculating MCI (mitochondrial complexity index)42. MCI is calculated as SA3/(16π2V2), where SA is the surface area and V is the volume of each mitochondrion (see details in the Methods section). c Representative 3D reconstruction of mitochondria extracted from serial EM images acquired by array tomography in Pdzd8f/f::CreERT2 MEFs infected with lentivirus carrying shControl or shFKBP8, and treated with or without 0.5 µM 4-OHT. Scale bars: 1 µm. d Quantification of MCI (mitochondrial complexity index)42. The data are presented as individual points on box plots, with the center indicating the median, and the 25th and 75th percentiles represented by the box. Whiskers extend to the minimum and maximum values. n = 63, 90, 54, and 52 mitochondria from 5, 5, 5, and 4 cells for the control, Pdzd8 cKO, Fkbp8 KD, and Pdzd8 cKO + Fkbp8 KD cells, respectively. Statistical analysis was performed using one-way ANOVA and Fisher’s LSD test. ****p < 0.0001, ***p = 0.0006, *p = 0.033. Source data are provided as a Source Data file.
