Fig. 5: Inhibition of NANS reverses the immunosuppressive microenvironment and suppresses the tumor growth of PCa in orthotopic transplanted mouse model. | Nature Communications

Fig. 5: Inhibition of NANS reverses the immunosuppressive microenvironment and suppresses the tumor growth of PCa in orthotopic transplanted mouse model.

From: Integrated proteogenomic characterization of localized prostate cancer identifies biological insights and subtype-specific therapeutic strategies

Fig. 5: Inhibition of NANS reverses the immunosuppressive microenvironment and suppresses the tumor growth of PCa in orthotopic transplanted mouse model.The alternative text for this image may have been generated using AI.

A Uniform manifold approximation and projection (UMAP) visualization of 29515 single cells from 6 mice tumor tissue samples, colored by cell type annotations. B UMAP visualization of epithelial cells, colored by cell type annotations. C Comparison of the Ro/e (the ratio of observed to expected cell numbers) in 4 epithelial cell subtypes before and after Nans knockout. P-values are determined using two-sided Chi-square test. D Bubble plot showing differentially activated hallmark pathways and sialic acid-related pathway among 4 epithelial cell subtypes. E Comparison of the Ro/e in all cell types before and after Nans knockout. P values are determined using two-sided Chi-square test. F UMAP visualization of macrophages, colored by cell type annotations. G Comparison of the Ro/e in 4 macrophage subtypes before and after Nans knockout. P values are determined using two-sided Chi-square test. H Dot plot depicting the expression of detected Siglec genes in each identified cell subtype, where dot size and color represent percentage of marker gene expression (pct. exp) and the averaged scaled expression (avg. exp. scale) value, respectively. I Heatmap depicting the enrichment of classical immune-related pathways in each identified macrophage subtype. J IF staining and quantification of sialylation level (SNA/MALII) and immune cells in murine tumor tissues of the sgNans and control group. n = 3 for each group. The scale bar represents 50um. Data are presented as mean ± SD. P values are determined using two-tailed Student’s t test. K Ex vivo imaging of murine tumor tissues of the sgNans and control group. n = 5 for each group. Data are presented as mean ± SD. P values are determined using two-tailed Student’s t test. L Comparion of tumor volume and weights between sgNans and control group. n = 5 for each group. Data are presented as mean ± SD. P values are determined using two-tailed Student’s t test. Source data are provided as a Source Data file.

Back to article page