Fig. 1: Identification of neurons associated with high sucrose preference.
a The mice consume water, as well as 2%, 12%, and 20% sucrose solutions, data are presented as mean ± SEM (n = 8 mice per group, water vs. 2% sucrose ***p = 1.93 × 10−13, water vs. 12% sucrose ***p < 1.0 × 10−15, water vs. 20% sucrose ***p = 6.0 × 10−15, two-way ANOVA). b The scatter plot and the bar chart showing the sucrose preference in the TBP, the sucrose preference was estimated at 0.63 ± 0.006 (group 1: n = 13 mice); 0.74 ± 0.008 (group 2: n = 24 mice) and 0.86 ± 0.009 (group 3: n = 13 mice), data are presented as mean ± SEM (1 vs. 2 ***p = 8.76 × 10−11, 1 vs. 3 ***p < 1.0 × 10−15, 2 vs. 3 ***p = 2.53 × 10−10, one-way ANOVA). c The scatter plot and the bar chart showing the place preferences in CPP, which were estimated at 0.60 ± 0.004 in group 1 (n = 13); 0.69 ± 0.007 in group 2 (n = 24), and 0.81 ± 0.006 in group 3 (n = 13), data are presented as mean ± SEM (1 vs. 2 ***p = 1.43 × 10−8, 1 vs. 3 ***p < 1.0 × 10−15, 2 vs. 3 ***p = 8.0 × 10−15, one-way ANOVA). d Heatmaps of the locomotor activity of CPP and linear regression scatter plot showing the correlation between place preference and sucrose preference. Pearson’s r (2-tailed), p < 1.0 × 10−15. e A plot showing time spent in the central chamber in the CPP, data are presented as mean ± SEM (one-way ANOVA, ns, no significant difference). f Representative heatmaps and a plot showing time spent in the light box of high (n = 13) versus low (n = 13) sucrose preference mice in the LDT, data are presented as mean ± SEM (***p = 1.64 × 10−13, two-tailed t-test). “Low” for low-sucrose-preference groups and “High” for high-sucrose-preference groups. TBP: two-bottle preference; CPP: conditioned place preference; UCP: unconditioned place (water); CP: conditioned place (12% sucrose); LDT: light/dark test. Source data are provided as a Source Data file.
