Fig. 5: snR107 restricts the amplitude and timing of meiotic gene expression.

a Scheme depicting the induction of ectopic meiosis by inhibition of the pat1-as allele (pat1-L95G) with 3-MB-PP1, which in turn activates the Mmi1 inhibitor Mei2 and hence prevents meiotic mRNA degradation. b Northern blots showing meiRNA and snR107 levels from total RNA samples isolated at different time points following addition of 3-MB-PP1 in wild type cells. Ribosomal RNAs served as loading control. V = vegetative cells; -N = nitrogen-starved cells. b = bases. c to e RT-qPCR analyses of mcp5+ and ssm4+ meiotic mRNA levels upon induction of meiosis in cells of the indicated genetic backgrounds (mean ± SD; n = 3 biological replicates for (11-510)∆, snR107∆, 3’SS_mut, 5’&3’SS_mut, snR107_kloopmut and snR107_ASE2mut strains; n = 4 biological replicates for wt and snR107_ASE1mut strains; normalized to total RNA concentration and relative to wt -N). Note that data for the wild type strain in c were replotted in d and e to ease comparison. Student’s t-test (two-tailed) was used to calculate p-values (relative to wt). NS = not significant. f Poly(A) + RNA-seq analyses of wild type and snR107_kloopmut cells upon meiosis induction (n = 2). Shown are the median expression profiles of the Mmi1 regulon¹⁰ and the corresponding heatmaps for each individual gene (relative to wt -N; log2 scale). V = vegetative cells; -N = nitrogen-starved cells. Source data are provided as a Source Data file.