Fig. 3: Comparisons to related structure.

a Overlay of the S. cerevisiae Rat1-Rai1-Rtt103 ternary complex structure reported here (in color) and the S. cerevisiae Rat1-Rai1 binary complex structure together with Pol II (gray)15. The Pol II structure is not shown here for clarity, but shown in Supplementary Fig. 4. b Overlay of Rai1 in the Rtt103 binding region. Strands β1 and β2 of Rai1 in the Rat1-Rai1-Pol II complex are disordered, and the Rtt103 binding site does not exist. c Overlay of the S. cerevisiae Rat1-Rai1-Rtt103 ternary complex structure reported here (in color) and the S. pombe Rat1-Rai1 binary complex structure (gray)9. d Detailed interactions in the Rat1-Rai1 interface. S. cerevisiae Rat1 is shown as an electrostatic surface, and Rai1 residues in the interface are shown as stick residues (green for S. cerevisiae Rai1 and gray for S. pombe Rai1). In all panels, the current structure of S. cerevisiae Rat1-Rai1-Rtt103 complex is shown in color, while previously published structures are in gray.