Table 1 Cryo-EM data collection, structure refinement and validation statistics
Saccharomyces cerevisiae Rat1-Rai1-Rtt103 complex | |
|---|---|
Data collection and processing | |
Magnification | 105,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 49.3 |
Defocus range (μm) | –0.6 to –2 |
Pixel size (Å) | 0.413 |
Symmetry imposed | C1 |
Image stacks (no.) | 6718 |
Initial particles images (no.) | 3,614,995 |
Final particle images (no.) | 552,608 |
Map resolution (Å) | 2.85 |
FSC threshold | 0.143 |
Map sharpening B-factor (Å2) | –123 |
Refinement | |
Number of protein residues | 1115 |
Number of metal ions | 1 |
Number of atoms | 8975 |
R.m.s. deviations | |
Bond lengths (Å) | 0.004 |
Bond angles (°) | 0.536 |
PDB validation | |
Clash score | 6 |
Poor rotamers (%) | 0 |
Ramachandran plot | |
Favored (%) | 98.5 |
Allowed (%) | 1.5 |
Disallowed (%) | 0.0 |
