Fig. 1: Identification of a peptide-independent CD4+ T cell restricted to HLA-DQ2.5. | Nature Communications

Fig. 1: Identification of a peptide-independent CD4+ T cell restricted to HLA-DQ2.5.

From: A naturally selected αβ T cell receptor binds HLA-DQ2 molecules without co-contacting the presented peptide

Fig. 1: Identification of a peptide-independent CD4+ T cell restricted to HLA-DQ2.5.The alternative text for this image may have been generated using AI.

a HLA-DQ2.5glia-ω1 and DQ2.5glia-ω2 tetramer co-staining on CD4+ T cells post tetramer-based magnetic enrichment of PBMC of wheat challenged coeliac disease donor #0648. Right plot represents expansions of individual clones, determined via index sorting, with TCR gene segment usage, CDR3 sequence and frequency shown for each clone. Box indicates cell designated G9 TCR. b HEK 293 T cells transiently co-transfected with G9 TCR and CD3γδεζ were stained with individual HLA-DQ2.5 tetramers presenting glia-α1, glia-α2, glia-ω1, or glia-ω2, respectively, or control HLA-DQ8glia-α1 tetramer. c Affinity measurement of G9 TCR against HLA-DQ2.5glia-α1/glia-α2/glia-ω1/glia-ω2/CLIP and HLA-DQ2.2glut-L1 interactions. HLA-DQ8glia-α1 was immobilised in the reference flow cell to control non-specific binding. For KD determination, all data were derived from two independent experiments in duplicate and curve fits using a 1:1 binding model. For each concentration, the points represent the mean and the error bars correspond to ± s.e.m.

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