Fig. 4: Altered inflammatory phenotype of p30-expressing cells.
From: Mutant CEBPA promotes tolerance to inflammatory stress through deficient AP-1 activation
a Fold change of expression of cytokine-expressing genes in p30 HPC-7 vs p42 HPC-7 and KO-52p42 vs KO-52EV during LPS treatment. Three biological replicates per timepoint were performed; center line at median, lower and upper hinges correspond to the first and third quartiles (25th and 75th percentiles). Upper whisker extends from the hinge to the largest value no further than 1.5 * IQR from the hinge (IQR, inter-quartile range). Lower whisker extends from the hinge to the smallest value at most 1.5 * IQR of the hinge. b Downregulated terms by GSEA (p30 vs p42, NES < 0, FDR < 0.05) of secreted proteins detected by mass spectrometry in supernatants of HPC-7 cells treated with LPS for 16 h. c Volcano plot of deregulated secreted proteins detected by mass spectrometry in p30 vs p42 supernatants treated with LPS for 16 h, highlighting cytokines. Unpaired two-sided Welch’s T test to generate p values and permutation based FDR, 250 randomizations. d Cytokine secretion levels detected by mass spectrometry over an LPS time course of 0, 8 and 16 hours, each column is a replica. e Ratio of colony number in LPS vs untreated conditions in WT and p30 hematopoietic progenitors. f HPC-7 cell count in the presence of 100 ng/mL LPS. Two-sided paired t test, ***p value = 0.0005. g Ratio of cell number of LPS vs untreated conditions in p42 and p30 HPC-7 cells at day 7. Violin plots show median at center and quartiles. h Percentage of total annexin V+ cells in WT and p30 hematopoietic progenitors after 4 days of LPS stimulation. i Percentage of total annexin V+ cells in p42 and p30 HPC-7 cells after 4 days of LPS stimulation. e–i n = 3 biological replicates, data represented as mean +/− SEM. For statistical significance, two-sided t tests were used, unpaired (e, g, i) or paired (h). Source data are provided as a Source Data file.
