Fig. 3: The figures of merit for PRM curves at 1, 10 and 100 ng of material.
From: Rapid assay development for low input targeted proteomics using a versatile linear ion trap
a, b The quantitative accuracy of matrix-matched curves on an ion trap of pooled IL-2 and IL-15 peptides in a background of dimethyl-labeled peptides. We generated three curves loading 100 ng, 10 ng, and 1 ng of material on-column. Box plots show the spread of measured values where the whiskers indicate 5% and 95% points, and the bold line indicates the median measurement. d Each dilution is a different color where colored dashed lines indicate the expected fold change. e, f The distribution of the Figures of Merit for the 1, 10, and 100 ng injections using PRM on the Q-LIT. All boxplots (a–c, e, f) are represented as median value. The box maxima extents to the 1st interquartile range (25th percentile), while the minima extends to the 3rd inner quartile range (75th percentile).
