Fig. 5: Four-input Boolean logic gates design. | Nature Communications

Fig. 5: Four-input Boolean logic gates design.

From: Engineering intercellular communication using M13 phagemid and CRISPR-based gene regulation for multicellular computing in Escherichia coli

Fig. 5

A 4-input AND gate. Top: 4-input ‘AND’ gate design: a combination of two-input ‘AND’ gates in sender cells and a two-input ‘AND’ gate in receiver cells. Bottom: Mean of green fluorescence (arb. units) ± SD of 6 independent replicates, measured by flow cytometry in the presence or absence of 1 mM IPTG, 1 % arabinose, 0.1 mg liter−1 aTc and 1 mM AHL inducers. B 4-input NAND gate. Top: 4-input ‘AND’ gate design: a combination of two-input ‘AND’ gates in sender cells, two inverters and a two-input ‘OR’ gate in receiver cells. Bottom: Mean of green fluorescence (arb. units) ± SD of 6 independent replicates, measured by flow cytometry in the presence or absence of 1 mM IPTG, 1 % arabinose, 0.1 mg liter−1 aTc and 1 mM AHL inducers. For both 4-input gates, the initial sender to receiver ratio was 3:1. Fold-change (FC) is calculated by dividing the ‘ON’ state by the ‘OFF’ state, while the quality score (Q) was calculated by dividing the lowest ‘ON’ state by the highest ‘OFF’ state. Source data are provided as a Source Data file.

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