Fig. 5: Inflammatory signatures are induced by different AAV serotypes and transgenes and depend on transgene expression. | Nature Communications

Fig. 5: Inflammatory signatures are induced by different AAV serotypes and transgenes and depend on transgene expression.

From: AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain

Fig. 5

A Combinations of promoters and transgenes used to transduce 3D spheroids. Heat maps visualizing the enriched GSEA terms in (B) astrocytes, (C) oligodendrocytes, and (D) neurons either in the total population (left panel) or only in cells positive or negative for transgene mRNA (middle and right panels respectively) against the Hallmark gene set (Molecular Signatures Database) compared to untransduced controls within each cell type using Kolmogorov-Smirnov test with FDR for multiple test correction. The ubiquitous CAG promoter is highlighted in red (all panels) whereas the non-expressed hAAT promoter is coloured in grey (Transgene- panel only). (NES, normalized enrichment score; *, adjusted P < 0.05; **, adjusted P < 0.01; ***, adjusted P < 0.001).

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