Fig. 8: AAV-induced pro-inflammatory signalling and apoptosis are dependent on p53. | Nature Communications

Fig. 8: AAV-induced pro-inflammatory signalling and apoptosis are dependent on p53.

From: AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain

Fig. 8

A Scheme of the experimental design for the transduction of hiPSC-derived astrocytes. Analysis of (B) P53-dependent genes, (C) ISGs and (D) cytokines and chemokines by qPCR in astrocytes at day 4 post-AAV2 transduction. Data are shown as mean ± SD between 3 independent experiments, each performed in technical duplicate. Statistical significance was determined by one-way ANOVA with Tukey post-hoc test. E Experimental design for the transduction of hiPSC-derived neurons. For A and E, schemes were partially created with Biorender. Costa, H. (2025) https://BioRender.com/u34k332. F Immunofluorescence staining of cC3 (red) in hiPSC-derived neurons at day 5 post-AAV9 transduction. Scale bar 100 µm. G Violin plot showing the quantification of cC3 staining. N = 31 FOVs for untransduced (UT), n = 36 FOVs for AAV9-CAG-GFP and n = 22 FOVs for AAV9-GFP + LV-GSE56 across cultures from 3 independent experiments, 3 coverslips per condition per experiment. Data points refer to individual fields of view. Statistical significance was determined by one-way ANOVA with Tukey post-hoc test. H LDH release assay performed on supernatants from neurons collected on day 5 post-AAV transduction. Data are shown as mean between 2 experimental replicates for all conditions except for AAV9 (n = 3). Source data are provided as a Source Data file.

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