Fig. 3: Graft-infiltrated T cells retain Th1 phenotype and cytotoxicity in Setdb1-deficient mice.
From: Targeting Setdb1 in T cells induces transplant tolerance without compromising antitumor immunity
a Heatmap of Th1-related gene expression in splenic CD4+ T cells from WT B6 and Setdb1f/fCd4-Cre mice before and after anti-CD3/anti-CD28 stimulation (72 h). b–h WT B6 and Setdb1f/fCd4-Cre mice were transplanted with BALB/c hearts. Allografts and serum were harvested on day 7 post-transplant, and graft-infiltrated mononuclear cells were purified and analyzed. b Representative histograms showing T-bet expression in T-cell subsets, as determined by flow cytometry. c Mean fluorescence intensity of T-bet in T-cell subsets (left panel) and percentage of T-bet expression by T-cell subsets (n = 6 per group). d Representative zebra plot of IFN-γ, TNF-α, granzyme B, and perforin expression as assessed by intracellular staining. e Percentage of IFN-γ and/or TNF-α expression (n = 6 per group). f Serum IFN-γ levels measured by CBA assay (n = 9 for WT, and n = 8 for Setdb1f/fCd4-Cre mice). g Percentage of granzyme B and/or perforin expression (n = 6 per group). h Mean fluorescence intensity of CD107a in T-cell subsets (n = 5 per group). Data are representative of three (b, d) independent experiments. Data are shown as means ± SEM of at least three (c, e, f, g, h) independent experiments. Unpaired Student’s-t test (two-sided). See also Supplementary Fig. 6 and Supplementary Fig. 7.
