Fig. 4: Setdb1-deficiency Leads to An Increased Proportion of Regulatory T Cells.
From: Targeting Setdb1 in T cells induces transplant tolerance without compromising antitumor immunity
a Heatmap of regulatory T cell (Treg)-related gene expression in splenic CD4+ T cells from WT B6 and Setdb1f/fCd4-Cre mice before and after heart transplantation (day 7). The WT B6 without heart transplantation group served as a control. b Representative zebra plots of splenic and graft-infiltrating Treg cells in WT B6 and Setdb1f/fCd4-Cre mice before and after heart transplantation (day 7). c Percentage of splenic and graft-infiltrating Tregs before and at 7 days after transplant (n = 9 per group). Unpaired Student’s t-test (two-sided). d, e Naïve splenic CD4+ T cells were purified from WT B6 and Setdb1f/fCd4-Cre mice, then stimulated with anti-CD3/anti-CD28 in the presence of IL-2 (10 ng/ml) and TGF-β (10 ng/ml) for 48 or 72 h. d Representative zebra plot of foxp3 expression determined by flow cytometry. e Percentage of Foxp3-expressing cells at 48 h and 72 h after being cultured in Treg-polarizing conditions (n = 5 per group). Unpaired Student’s t-test (two-sided). f Gene set enrichment analysis (GSEA) of splenic CD4+ T cells from WT B6 and Setdb1f/fCd4-Cre mice before and at 7 days post-transplant. g BALB/c heart graft survival in Setdb1f/fCd4-Cre mice with/without anti-CD25 injection (n = 6). Log-rank (Mantel-Cox) test. h BALB/c heart graft survival in WT B6 and Setdb1f/fFoxp3-Cre mice (n = 3). ns: no significance; log-rank (Mantel-Cox) test. Data are representative of at least four (b, d) independent experiments. Data are shown as means ± SEM of at least three (c, e, g, h) independent experiments.
