Fig. 4: The Hr-like domains of BTSL2 and BTS are redox-active with O2 and H2O2. | Nature Communications

Fig. 4: The Hr-like domains of BTSL2 and BTS are redox-active with O2 and H2O2.

From: Iron-sensing and redox properties of the hemerythrin-like domains of Arabidopsis BRUTUS and BRUTUS-LIKE2 proteins

Fig. 4

Redox titrations monitored by UV-visible absorbance spectroscopy of (a, b) BTSL2-N and (d, e) BTS-N (MBP fusion proteins, see Fig. 3). Black line: ‘as-isolated’ proteins purified in air (a, d) or end point of the titration (b, e). Grey line: fully reduced with dithionite in an anaerobic chamber and desalted. Colour lines: titration with O2 (a, d, blue lines) or H2O2 (b, e, orange-red lines). c, f The difference in absorbance at 359 nm as a function of the oxidant to iron ratio based on the spectra in (a, b) for BTSL2-N, and spectra in (d, e) for BTS-N.

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