Fig. 2: Glucose contribution to TCA cycle metabolites in oHSV-infected cells.
From: IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunity
Effect of oHSV infection on central carbon metabolism. a Schematic of glucose metabolites in glycolysis and TCA cycle. Filled purple circles indicate the labeled carbons from [U-13C] glucose in the first round in TCA cycle. [Created in BioRender. Sahu, U. (2025) https://BioRender.com/s86n709]. GBM12 cells infected with oP10 (P) at MOI = 0.01 for 12 h were cultured in glucose-free media for 4 h followed by supplementation with U-13C glucose and 2% dialyzed FBS for 6 h were analyzed by LC/MS (n = 6/group) for the indicated metabolites (M + n, where n refers to the number of labeled carbon atoms in the metabolite). Total percentage of the indicated metabolite labeled with 13C for Glucose-6-Phosphate/Fructose-6-Phosphate (b) and 3-Phosphoglycerate/2-Phosphoglycerate (c) (n = 6). d Pyruvate kinase (PK) activity in nmol/min/mL (n = 3) and e Pyruvate dehydrogenase (PDH) activity in nmol/min/mL in lysates from control (C) or P-treated GBM12 cells (n = 3). f Concentration of total Acetyl-CoA (ng/µL) in control and oHSV-infected GBM12 cells (n = 3). Percentage of Citric acid (g), Succinic acid (h), and Malic acid (i) labeled with 13C from U-13C glucose (n = 6). Data are shown as mean ± S.D. (n ≥ 3 independent replicates). (Two-way ANOVA, unpaired two-tailed Student’s t-test). MOI = Multiplicity of infection. Source data are provided as a Source Data file.
