Fig. 8: Impact of Ferroptosis on tumor immune environment after virotherapy.

Single-cell sequencing analysis of CD45⁺ cells isolated from 005 glioma-bearing hemispheres of mice treated with oP10 5 days after virotherapy. a UMAP visualization of the composition of the annotated cells from virus or virus and ferrostatin-1 treated animals, each color representing a different cell cluster. Cell number: 10504 for P; 12427 for P+Fer-1. b Cell–cell interaction network among the major cell clusters in P and P+Fer-1 group. Line width represents number of interactions between the two interacting cell types. c Heatmap comparing differential number of interactions and interaction strength between the immune cell populations in tumors of P and P+Fer-1 treated mice. P+Fer-1 has fewer interactions of all cell types to T cell cluster. d UMAP represents subclusters of T cells, each color denoting an individual cluster. Cell number: 664 for P; 801 for P+Fer-1. e A composite bar graph of the T cell cluster representing altered T cell subclusters in P and P+Fer-1. f Marker genes used to annotate T cell subclusters. Dot size depicts the % of cells of a cluster expressing the given gene, and color intensity indicates the expression level of the gene by that cluster. g, h Mouse glioma 005-OVA tumors were established in C57BL/6 mice. Seven days later, tumor-bearing mice were treated with P±Fer-1. Anti-tumor and antiviral-specific T cells were analyzed by OVA tetramer (g) and HSV gB tetramer (h) staining in tumors 21 days post-tumor implantation (C and P/Fer-1: n = 5; P: n = 3), respectively. Data = Mean cell count ± S.D from n = 3–5 mice per group. (One-way ANOVA). C control, P oP10 infected. Source data are provided as a Source Data file.