Fig. 4: Durotaxis of Walker cells is independent of integrins.

a Example pictures of cells at an earlier (top panels) and later (middle panels) time point, migrating in stiffness gradient channels under control conditions or in the presence of integrin inhibitors (10 µg mL^–1 β1-integrin blocking antibody + 10 µM Cilengitide). The cellular membrane and the nucleus are pseudocoloured. Scale bar, 50 µm. The bottom panels show temporal colour-coded projected tracks of the cell each. b Kymographs of the cells shown in (a) over the whole distance. Scale bar, 100 µm (horizontal), 60 min (vertical). c Quantification of cell speed. n = 103 cells for control and 98 cells for inhibitor conditions; two-tailed Mann–Whitney test; *P = 0.01366. d Quantification of durotaxis index. n = 103 cells for control and 98 cells for inhibitor conditions; two-tailed Mann–Whitney test; P = 0.12506. Box plots centres are the mean with bounds representing the IQR and whiskers extending to a maximum of 1.5× IQR beyond the box. Source data are provided as a Source Data file.