Fig. 5: Structure of K784T mutant. | Nature Communications

Fig. 5: Structure of K784T mutant.

From: Multimeric transcription factor BCL11A utilizes two zinc-finger tandem arrays to bind clustered short sequence motifs

Fig. 5: Structure of K784T mutant.The alternative text for this image may have been generated using AI.

A Two molecules of K784T bind one DNA molecule. B Superimposition of two mutant molecules. C Molecule D interacts with base pairs 3-9. D Molecule E interacts with base pairs 10-14. (E) N756 of molecule D interacts with A4. (F) N756 of molecule F interacts with A11. G ZF5 of molecule D (including Thr784) recognizes C5 to T9. H ZF5 of molecule E (also including Thr784) recognizes T12 to T14. We observed that the AlphaFold3-predicted K784T mutant binds in a similar way to molecule D, and the interactions involving Asn756-A4 and Arg787-G8 are shifted by one base pair, now occurring at A3 and G7. There is no equivalent to molecule E is predicted.

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