Fig. 4: CpaF phylogeny coupled to structural prediction reveals three CpaF domain architectural classes.
From: Conformational changes in the motor ATPase CpaF facilitate a rotary mechanism of Tad pilus assembly
A Phylogenetic tree of 292 CpaF orthologs and 3 archaellar ATPases generated by RAxML maximum-likelihood analysis. Branches are colored according to bacterial phyla, while nodes with rapid bootstrap values greater than or equal to 80 are indicated by black circles. Black branches depict the archaellar ATPase outgroup. The tip of each leaf is associated with one of three classes of CpaF domain architecture, based on the predicted structure of each sequence from the AlphaFold Protein Structure Database. The three CpaF domain architecture classes are depicted in the legend along with the number of orthologs in each class. Accession numbers of each CpaF ortholog can be found in Supplementary Data 1 and the tree can also be viewed at the following link: https://itol.embl.de/tree/132204251252204561721920806. FHA forkhead-associated domain, IDR intrinsically disordered region, 3HB three-helix bundle, NTD N-terminal domain, CAD C-terminal ATPase domain. B Representative CpaF orthologs from each class and their AlphaFold predicted structures retrieved from UniProt, colored according to the domain architecture in (A). UniProt accession numbers: E1CIZ1 (Aggregatibacter actinomycetemcomitans TadA), Q9L714 (Caulobacter crescentus CpaF), and E3HIW0 (Achromobacter xylosoxidans CpaF).
