Fig. 8: Construction of the neuromuscular tissues using human induced pluripotent stem cell (hiPSC)-derived myogenic progenitor cells (MPCs) and motor neurons (MNs).

a Schematic of strategies in personalized tissue fabrication with acoustofluidic bioassembly using hiPSC-derived MPCs and MNs. b Immunofluorescence images of hiPSC-derived MPCs after differentiation, stained with MF20 (green)/MyoD (red)/DAPI (blue) and Desmin (green)/PAX7 (red)/DAPI (blue). c Immunofluorescence image of TUJ1 (green)/ISL1 (red)/DAPI (blue) of hiPSC-derived MNs after differentiation. d Brightfield images of the neuromuscular tissues of hiPSC-MPC + MN R and hiPSC-MPC + MN AB groups at day 0 and 5. e Immunofluorescence images of the tissues of the hiPSC-MPC + MN AB groups at day 5 stained with MF20 (green)/TUJ1 (red)/DAPI (blue) and desmin (green)/TUJ1 (red)/DAPI (blue). f Alignment index of the cells in the hiPSC-MPC + MN R (R) and hiPSC-MPC + MN AB (AB) groups at day 5 (n = 3 biologically independent samples; **P < 0.01 versus R). a.u., arbitrary units. Scale bars in (b–e) represent 50 μm, 50 μm, 100 μm, and 25 μm, respectively. Data is presented as mean ± S.D., and statistical differences were determined with an unpaired, two-sided t-test. Source data are provided as a Source Data file.