Fig. 3: Amplified ethylene-activated signaling causes root coiling of crw1 when grown in water.
From: Mechanosensing antagonizes ethylene signaling to promote root gravitropism in rice
a, b Gene oncology pathway enrichment of differentially expressed genes (DEGs) between crw1 and WT roots. WT and crw1 seedlings were grown in water for 2 days and whole roots were sampled for RNA-seq analysis. An adjusted p-value of ≤0.05 in multiple tests and an absolute log2fold change value ≥ 1 were used as the thresholds for determining significant differences in gene expression. a Top 25 pathways sorted based on the enrichment factor and p-value. b Expression levels of DEGs in the ethylene-activated signaling pathway. c–f WT, crw1 and OsCRW1 knockout lines were grown in water with or without 10 μM 1-Methylcyclopropene (1-MCP) for 2 days. c Lugol’s staining of amyloplast. d DR5rev::VENUS signal in root tips. e Root phenotypes. f Root depth. Data shown are mean ± SD, n = 6 in the control group and 7 in the 1-MCP group. Significant difference from WT was determined by two-sided Student’s t-test. Scale bars are 100 μm in (c and d) and 1 cm in (e). Data and images shown are representative results of three independent experiments with similar results. Source data are provided as a Source Data file.
