Fig. 7: Mechanosensing induced Ca2+ signaling antagonizes ethylene signaling to maintain root gravitropism in crw1.
From: Mechanosensing antagonizes ethylene signaling to promote root gravitropism in rice
a, b WT and crw1 (both expressing NES-YC3.6) were grown in water or different soil for 2 days. a The NES-YC3.6 fluorescence in the root caps. b Quantification of NES-YC3.6 fluorescence in the root caps. c–j WT and crw1 expressing various reporters were grown in 0.4% and 0.8% agar with or without 100 μM LaCl3 for 2−3 days. (c) Cytosolic Ca2+ in the root caps detected by NES-YC3.6. d Quantification of NES-YC3.6 intensity. e Lugol’s staining of root caps. f DR5rev::VENUS fluorescence of root tips. g Root phenotypes. h, i Level of OsEIL1 and OsEIL2 proteins in WT and crw1 roots by immunoblot. OsEIL1-Flag and OsEIL2-Flag proteins were detected by Flag antibody (anti-Flag) and the Actin (anti-Actin) was used as a loading control. j Transcriptional level of OsERF82 in roots by Q-PCR. OsHistone and OsActin were used as the internal reference genes. Data are means ± SD; n = 12 in (b), n = 15−19 in (d), and n = 4 in (j). Different letters above bars indicate a significant difference at P < 0.05 (two-way ANOVA followed by Tukey’s test). Scale bars are 1 cm in (g) and 100 μm in (a, c, e, f). Data and images shown are representative results of three independent experiments with similar results. Source data are provided as a Source Data file.
