Fig. 4: Analysis of SMXL7pro:SMXL7d53-mVenus, WOX4pro:D14/d14, WOX4pro:D14/max2, and WOX4pro:D14/max1 plants.
From: Strigolactones optimise plant water usage by modulating vessel formation
a–d Toluidine blue-stained hypocotyl cross-sections from 5-week-old wild type (a, b) and d14 (c, d) plants carrying a SMXL7pro:SMXL7d53-mVenus (b) or WOX4pro:D14 (d) transgene. e, f Quantification of vessel element number per section (e) and mean vessel element area size per section (f) comparing wild type, d14, lines carrying WOX4pro:D14 transgenes in the d14 background and lines carrying a SMXL7pro:SMXL7d53-mVenus transgene. n = 11 (WT), 13 (SMXL7pro:SMXL7d53-mVenus/WT), 12 (d14) and 9 (WOX4pro:D14/d14) plants for each genotype. This assay was conducted in parallel with the brc1 vessel element analysis, and both share the same wild-type data. g, h Basic Fuchsin-stained hypocotyl cross-sections from 2 week-old 35Spro:XVE > >miRNA165a transgenic plants in wild type (g) and SMXL7pro:SMXL7d53-mVenus background (h), following miRNA165a induction by 17β-Estradiol (17-β) in 5-day-old seedlings. i, j Quantification of vessel element number per section (i) and mean vessel element area size per section (j). n = 17 (WT, Mock), 22 (WT, 17-β), 17 (SMXL7pro:SMXL7d53-mVenus, Mock) and 25 (SMXL7pro:SMXL7d53-mVenus, Mock, 17-β) plants for each group. k–o Toluidine blue-stained hypocotyl cross-sections from five-week-old wild type (k), max2 (l), max2 plants carrying a WOX4pro:D14 transgene (m), max1 (n) and max1 plants carrying a WOX4pro:D14 transgene (o). p, q Quantification of vessel element number per section (p) and mean vessel element area size per section (q). n = 8 (WT), 11 (max2), 11 (WOX4pro:D14/max2), 10 (max1) and 13 (WOX4pro:D14/max1) plants for each genotype. Statistical groups are indicated by letters and were determined by a one-way ANOVA with post-hoc Tukey-HSD (95% CI). Vessel elements were automatically identified using ImageJ and subsequent manual correction (marked in red) (a–d, k–o). Scale bar represents 50 μm. Source data are provided as a Source Data file. For box plot definition, please see “Statistics and reproducibility” section.
