Fig. 5: Comparison of peptide patterns in dense-core plaques.
From: Chemical imaging delineates Aβ plaque polymorphism across the Alzheimer’s disease spectrum
a, b Representative cored (CP) and coarse-grained plaque (CGP) specific average mass spectra obtained for multiple ROI/plaque type in one sporadic Alzheimer’s disease (sAD) and one familial Alzheimer’s disease (fAD) patient. c, d OPLS-DA score plots for comparison of CP and CGP spectral data across sAD (c) and fAD (d) showing separation of the populations. e OPLS-DA of CP in between sAD and fAD indicated partial separation of fAD CP and sAD CP, (f) while no separation was observed for CGP in between sAD and fAD. g Representative single ion images of statistically significant Aβ peptides obtained from one patient per group (sAD, fAD). Intensity scale: rel. intensity in %. Scalebar: 50 μm. h Corresponding bar plots showing univariate comparison of single ion intensities across respective plaque types. Plots indicate single plaque values mean±SD. Number of patients: n = 12 sAD and n = 6 fAD; number of plaques per patient and plaque type N = 5–15. Univariate analysis was performed using Kruskall-Wallis test with two-sided Dunn’s correction: Aβ5-42 fAD-CP vs. fAD-CGP p = 0.0002; Aβ1-42 fAD-CP vs. sAD-CP p = 0.0003.; (***p < 0.001, ****p < 0.0001).
