Fig. 2: Photoreversible phase-state control of Azo-modified DNA condensates at constant temperatures.
From: Remote-controlled mechanical and directional motions of photoswitchable DNA condensates
Time-sequential images acquired by phase contrast (PC) microscopy of a a gel-to-liquid phase change under UV at 46°C and b a UV-induced liquid-to-dissociated state change (‘Original’ to ‘0 min’) reversed by additional Vis irradiation at 53°C. a (ii), (iii), b (ii): controls. c Coalescence of the photoreversed DNA liquid condensates captured in (b). Time-sequence started 52 min after switching to Vis irradiation. Yellow triangles mark coalescing condensates. d Procedures and conditions of e fluorescence recovery after photobleaching (FRAP) of Azo-modified DNA gel-state condensates at 35°C in a dark environment (‘Dark’), UV irradiation followed by the dark (‘UV→Dark’), and consecutive UV and Vis followed by the dark (‘UV→Vis→Dark’). Each solid line connects the mean values at specific elapsed times for clarity. Shaded areas indicate S.D. \((n\ge 4)\). See Supplementary Fig. 3d for the individual data points. d, e Shaded rectangles in pale green highlight the post-bleaching irradiation conditions. Irradiation intensities: (a) 1.1 mW cm–2 in the first 60 min of UV, 0.60 mW cm–2 in the additional irradiation; (b, c, e) 8.9 mW cm–2 (UV), 10.4 mW cm–2 (Vis). Scale bars: (a, b, d) 50 µm; (b inset) 10 µm; (c) 20 µm. Source data are provided as Source Data files.
