Fig. 4: Live barcode balancing using WarpDemuX.
From: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing
a Principle of live barcode balancing. Raw signal data is accumulated and, once the start of a poly(A) tail is detected, submitted to the WarpDemuX live classifier. Based on its classification, a decision is taken to either retain or reject the barcode, resulting in increased read turnover when rejecting. b Channel assignments for live barcode balancing during the experiment, with 40 channels per WarpDemuX balancing strategy for comparative analyses. The no-WDX channels served as internal control. c Cumulative distribution plot of duration of WarpDemuX barcode classification itself. d Histogram for number of nucleotides that passed through the pore from the start of poly(A) until the rejection decision. e Bar plot of the number of adapter counts per pore per balancer, colored by the decision taken after classification of the adapter. (f) Bar plot showing the total number of reads recovered with- and without barcode balancing (40 channels each). Inset shows enrichment for low abundance samples. Here we show the results for the adapter count balancing strategy, while results for the other strategies are depicted in Supplementary Fig. 25. Source data are provided as a Source Data file.
