Fig. 7: PRPS2 enhances the methionine metabolic cycle and upregulates SAM synthesis. | Nature Communications

Fig. 7: PRPS2 enhances the methionine metabolic cycle and upregulates SAM synthesis.

From: PRPS2 enhances RNA m6A methylation by stimulating SAM synthesis through enzyme-dependent and independent mechanisms

Fig. 7: PRPS2 enhances the methionine metabolic cycle and upregulates SAM synthesis.

a Schematic of 13C stable isotope-labeled carbon flow from 13C6-glucose or 13C1- methionine through the methionine metabolic cycle. b 13C-labeled ATP elimination in 1-, 4-, and 6 h timeframes measured by targeted LC-MS/MS from PRPS2 or PRPS1 ectopic stable expression cell line H1299. c–h Peak areas of 13C-labeled metabolites through the methionine metabolic cycle. The data were monitored after a 6 h timeframe of 13C6-glucose (red dots) or 13C1-methionine (cyan dots) treatments. The data in (b) were plotted as the mean ± SDs of biological triplicates. The data in (c–h) were plotted as the mean ± SDs of six biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 for multiple comparisons calculated using one-way ANOVA with Tukey’s HSD test in (b–h).

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