Fig. 3: Biophysical model with mechanical feedback between stiffness and cell fate recapitulates imaging results.
From: Self-propagating wave drives morphogenesis of skull bones in vivo
A Schematic of the model. B Dynamics of the osteogenic front and the tracked cells for the theoretical model, the ex vivo live imaging experiments (N = 4), and in vivo fixed tissue images obtained by comparing bone sizes between E13.5 (n = 7) and E14.0 (n = 7). Shaded areas indicate SEM for the ex vivo data. C Cell velocity for the theoretical model compared to the velocities obtained from the ex vivo tracked cells over 6 h (Fig. 1H). Error bars show standard deviations in both horizontal and vertical directions. D The simulated fraction of osteoblasts shows a sigmoidal profile across space that travels as a wave. The horizontal axis shows the position along the medial-lateral axis, where the origin represents the initial position of the osteogenic front, here defined to be the location where ϕ = 1/2.
