Fig. 2: Loss of tRNA wobble modification sensitizes cancer cells to mTORC1 pathway inhibition in vitro and in tumors.
From: mTORC1 cooperates with tRNA wobble modification to sustain the protein synthesis machinery
a–c Fold change in cell number of single cell-derived clonal Ctu1 KO or control EPP2 cells expressing sgRNAs against (a) Raptor, (b) Rictor ± rapamycin [12.5 nM] and (c) Rheb after 3 days in culture. d Fold change in cell number of Ctu1 iKO EPP2 cells after 3 days in culture ± GDC-0941 [1 µM] or MK-2206 [2 µM]. a–d Data are represented as mean ± SD (n = 3 technical replicates). e Weight of orthotopic pancreatic tumors from Ctu1 iKO or control EPP2 cells in C57BL/6 J Rag2−/− mice after 9 days treatment with rapamycin [5 mg/kg/day] or vehicle. Centre line represents median, upper and lower bounds of the box 75th and 25th percentiles, whiskers min to max (control ± rapamycin n = 6, Ctu1 iKO ± rapamycin n = 7); p values were calculated by unpaired two-tailed t-test with Welch correction. Source data are provided as a Source Data file.
