Fig. 5: mTORC1 and tRNA wobble enzymes converge on sustaining ribosomal protein synthesis.
From: mTORC1 cooperates with tRNA wobble modification to sustain the protein synthesis machinery
a, b Codon-specific changes in ribosome A site occupancy in (a) control and (b) Ctu1 iKO EPP2 cells after 2 h ± torin 1 [300 nM], analyzed by diricore. VAA codons cognate for U34 wobble tRNAs are highlighted. Data are represented as mean (n = 6 independent experiments); p values were calculated by out-of-frame analysis and are shown for significant increases and for all U34-enzyme-dependent codons. c, d Changes in global and ribosomal protein abundance in the newly synthesized proteome of EPP2 cells after 5 h ± (c) torin 1 [50 nM] and (d) rapamycin [50 nM], quantified by the QuaNPA workflow (n = 3 experimental replicates). In box plots, centre line represents median, upper and lower bounds of the box 75th and 25th percentiles, whiskers 10th to 90th percentiles. e, f Correlation of mRNA AAA codon usage deviation with changes in the newly synthesized proteome of EPP2 cells after 5 h ± (e) torin 1 [50 nM] and (f) rapamycin [50 nM]. Shown are protein groups whose synthesis is particularly dependent on Ctu1. Data are represented as median and interquartile range. Source data are provided as a Source Data file.
