Fig. 6: Ctu1 promotes efficient synthesis of ribosomal proteins in a codon-dependent manner.
From: mTORC1 cooperates with tRNA wobble modification to sustain the protein synthesis machinery
a, b Scatter plot comparing the newly synthesized proteome of EPP2 cells after 5 h mTORC1 inhibitor ± Ctu1 iKO (Ctu1 effect) or Ctu1 iKO ± 5 h mTORC1 inhibitor (inhibitor effect). mTORC1 inhibitors were a) torin 1 [50 nM] and (b) rapamycin [50 nM]. Newly synthesized proteomes were quantified using the QuaNPA workflow (n = 3 experimental replicates). c–h Expression of HA-tagged ribosomal proteins (c, d) Rpl29, (e, f) Rps25 and (g, h) Rps3 in Ctu1 iKO EPP2 cells. Expression of wild type (WT) and VAA-to-VAG mutant (VAG Mut.) ribosomal protein variants was analyzed after 40 h + rapamycin [50 nM], MG132 [200 nM] by quantitative immunoblotting. Codon usage deviation of wild type proteins is indicated. Data are normalized to WT sgRNA controls and represented as mean ± SEM (n = 4 independent experiments); p values were calculated by two-tailed one sample t-test with a hypothetical mean of 1. Source data are provided as a Source Data file.
