Fig. 8: mTORC1 cooperates with tRNA wobble enzymes to sustain translational capacity.
From: mTORC1 cooperates with tRNA wobble modification to sustain the protein synthesis machinery
a–g Puromycin (puro) incorporation assay in U34-enzyme-deficient EPP2 cells after 16 h inhibitor treatment, analyzed by quantitative immunoblotting. Asterisk denotes an unspecific band. a, b Ctu1 iKO ± torin 1 [300 nM]; (c) Ctu1 iKO ± GDC-0941 [1 µM], MK-2206 [2 µM], rapamycin [12.5 nM]; (d, e) Elp3 iKO ± torin 1 [300 nM]; (f, g) Ctu1/Elp3 iDKO ± torin 1 [300 nM]. Data are normalized to sgRNA controls (dashed line) and represented as mean ± SEM (b, e) DMSO n = 8, torin 1 n = 4, (c, g) n = 4 independent experiments). p values were calculated by two-tailed one sample t-test with a hypothetical mean of 1. h Puromycin incorporation assay in Elp3 iKO EPP2 cells after torin 1 [300 nM] treatment for the indicated periods, analyzed by immunoblotting. i, j Puromycin incorporation assay in Elp3 iKO EPP2 cells after 40 h torin 1 [300 nM] followed by 1 h washout of torin 1, analyzed by quantitative immunoblotting. Data are normalized to sgRNA control + torin 1 and represented as mean ± SEM (n = 5 independent experiments); p values were calculated by two-tailed unpaired t-test with Welch correction. Source data are provided as a Source Data file.
