Fig. 2: Shoot BR affects root genes that respond to carbon availability and regulate LR formation.
a An illustration depicting the scientific question. b Normalized counts of the CPD transcripts in the biological replicates corresponding to the four grafting combinations, WT/WT, WT/cpd, cpd/cpdun, and cpd/WT. c PCA analysis displays the biological replicates as above. d A volcano plot displaying –log10 p values vs log2 fold change (FC) of genes in a comparison between cpd/WT and WT/WT. Differentially expressed genes (adjusted p < 0.05, −0.8 > log2FC > 0.8) are colored. e A Venn diagram demonstrating the number of genes with higher expression in roots of WT/cpd, cpd/cpdun, and cpd/WT as compared to WT/WT. Note the high overlap between WT and the cpd (322 genes, divided into 168 and 154 genes in the Venn diagram) upon grafting WT roots to cpd. Common genes across all three pairwise comparisons indicate that this shoot-derived gene regulation depends on root BR. f Functional enrichment of the 322 common genes shown in (e). g LR formation related genes that are rescued in cpd roots by shoot BR were selected from comparing WT/cpd vs cpd/cpd. Adjusted p value is depicted by color and log2FC is noted in parenthesis.
