Fig. 5: R1 and NLS of CgPho4 confer stronger activity than their counterparts in ScPho4.
A Pho4 chimeras (black), endogenous ScPho4 (green), and CgPho4 (blue) were plotted based on their activity with and without Pho2. The solid line indicates equal activity (independent of Pho2). B Heatmap showing the difference in activity between the CgPho4 region(s) and the counterpart of ScPho4 measured in the ScPho4 background. The upper half shows the difference with Pho2 and the lower half without Pho2. The triangles (e.g., a) show the difference between single regions while the squares on the top show the differences when swapping two regions (see key to the right). Red color indicates a higher activity of the region from CgPho4 than the counterpart in ScPho4 and blue color indicates decreased activity. C A subset of the chimeras were shown with their APHO2 and Apho2∆ values. Bars represent the means; lines are 95% confidence intervals based on bootstrap; plus signs are individual data points (n = 6, biological replicates), which were not shown for the endogenous ScPho4 and CgPho4 (n = 36, biological replicates). Vertical dotted lines equal the endogenous ScPho4 level. D Epistasis analysis of CgPho4 R1, AD, and NLS. A linear model was fit to the data with all two and three-way interactions. Shown are the individual region and interaction coefficient estimates (bar and point) for the CgPho4 region(s). Line ranges centered on the estimates (bar and point) indicate the standard errors of the coefficients as calculated by the linear model fit. Dark gray indicates the term is significant at 0.05 level by student’s t-test after Holm-Bonferroni correction.
