Fig. 4: Phospholipid compositions and membrane fluidity of LDs purified from thermosensitive fusion mutants.

a Molar percentages of major classes of glycerol phospholipids of LDs purified from WT and thermosensitive fusion mutants. Y axis is on the log10 scale. b, c Relative levels of PC and PE subspecies of purified LDs. PC36:2(18:1/18:1) refers to a PC subspecies with two C18:1 fatty acyl chains, etc. The molar percentages of triplicate LD samples of each mutant were normalized against the mean molar percentage of WT LDs, which was arbitrarily valued as 1. Data were exported as heat maps. d, e Molar percentages of PC and PE subspecies grouped according to chain lengths. The coloring scheme in (c-e) is the same as that in (a, b). In (a, d, e), each data column is mean ± SEM. N = 3 biological replicates. ns, P ≥ 0.05. *, P < 0.05. **, P < 0.01. ***, P < 0.001. Unpaired two-sample two-tailed t-test. In (a), differences uncorrelated with fusion capabilities or without significance are not labeled. f Flowchart of FRAP on purified GFP::DGAT-2-labeled LDs. g Representative FRAP images of LDs of WT and fusion mutants. White box, FRAP area. h Time course of FRAP of GFP::DGAT-2 fluorescence. Each line is the average of at least 20 individual FRAP data lines. i T-half values of FRAP. N = 20, 22, 23, 22, 25, and 20 LDs, respectively. Data are presented as box-whisker plot, which shows the lower extreme (10%), lower quartile, median, upper quartile, upper extreme (90%), and mean (diamond) values. For statistical tests, ns, P ≥ 0.05. ***, P < 0.001. Unpaired two-sample two-tailed t-test. All exact P-values and source data of (a–e, h, i) are provided as Source Data file.