Fig. 3: GABA release activates GABABRs with age-dependent differences.
From: Phylogenetic divergence of GABAB receptor signaling in neocortical networks over adult life
A Immunofluorescence for GABAB1 (green) and NeuN (magenta) in rat and human neocortex, with layers (dashed lines) and depth (arrow). Scale = 100 μm. B GABAB1 intensity normalised to cortical depth for 1 (black, n = 5), 6–8 (grey, n = 3) and 12–14-month-old (grey, n = 3) rats, with SEM (dotted lines). C GABAB1 intensity for human (magenta, 6 patients) and adult rat (black, 6–14-months). D Schematic of IPSC recordings, with control and CGP from L2/3 (black) and L5 (blue). E IPSC amplitudes in 1 (L2/3: 19 cells/11 rats; L5: 17 cells/8 rats), 6–8 (L2/3: 22 cells/13 rats; L5: 17 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats. E’ IPSC amplitude for adult rats and humans (L2/3: 19 cells/13 cases; L5: 8 cells/5 cases). F IPSC 20–80% rise-time in 1-month (L2/3: 18 cells/10 rats; L5: 5 cells/5 rats), 6–8-month (L2/3: 15 cells/13 rats; L5: 7 cells/6 rats), and 12–14-month-old (L2/3: 11 cells/5 rats; L5: 3 cells/2 rats) rats. Rise-time could not be measured for all IPSCs. F’ 20–80% rise-time in adult rat and humans (L2/3: 14 cells/10 cases; L5: 6 cells/4 cases). G IPSC decay time-constant from 1 (L2/3: 18 cells/10 rats; L5: 6 cells/5 rats), 6–8 (L2/3: 15 cells/13 rats; L5: 7 cells/6 rats), and 12–14 (L2/3: 11 cells/5 rats; L5: 3 cells/2 rats) rats (L5: 1 vs. 12–14 months, p = 0.035, L5: 6–8 vs.12–14 months, p = 0.0013; 12–14-month: L2/3 vs. L5: P = 0.0048). G’ Decay time-constants of adult rat and human (L2/3: 17 cells/10 cases; L5: 5 cells/5 cases). H IPSC charge-transfer 1 (L2/3: 19 cells/11 rats; L5: 17 cells/8 rats), 6–8 (L2/3: 20 cells/13 rats; L5: 15 cells/13 rats), and 12–14-month-old (L2/3: 12 cells/5 rats; L5: 11 cells/5 rats) rats. H’ IPSC charge-transfer for adult rat and humans (L2/3: 22 cells/13 cases; L5: 8 cells/5 cases). Data shown as boxplots (25–75% range) with median indicated and whiskers indicating maxima/minima, showing individual points. Statistics from LMM with 2-sided Tukey post-hoc tests: *P < 0.05, **P < 0.01; ***P < 0.001. Source data are provided as a Source Data file.
