Fig. 2: Design and construction of the genome-wide, multi-targeted tomato CRISPR library. | Nature Communications

Fig. 2: Design and construction of the genome-wide, multi-targeted tomato CRISPR library.

From: Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level

Fig. 2

a Schematic visualization of the 10 sub-libraries, detailing the main gene families targeted, the number of genes targeted, and number of sgRNAs designed. MC, mitochondrial carrier. Numbers in bold and in brackets in ‘Genes’ column represent the number of genes targeted and the total number of genes (respectively) in aforementioned groups. b Bar chart of the number of genes targeted (black) versus the total number of genes in each sub-library (gray). c Distribution of the number of target genes per sgRNA in the library. d Pie charts of the number of mismatches between the designed sgRNAs and their target genes for sub-library 1 (top) and the entire library (bottom). e The frequency of numbers of sgRNA reads in sub-library 1 as determined by deep sequencing. Coverage was compared to the number of sgRNAs theoretically present in the library designed in silico. f Skewness (left axis, orange) and coverage (right axis, blue) of each sub-library. Orange and blue dotted lines mark the high-quality thresholds for skewness (−1, 1) and coverage (95%), respectively. Skewness for deep sequencing results were calculated as 3*(mean-median)/std dev. Source data are provided as a Source Data file.

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