Fig. 4: Single-cell RNA-seq analysis revealed infiltration of activated macrophages in the kidney cortex following DNA damage in PTECs.

A UMAP plots of the single-cell data classified by cell clusters (left), relative proportions of cells in the I-PpoI (Tg) and WT mice (middle left) and sample information (middle right). Expression of selected marker genes for each cell classification (right). The color of the dot is proportional to the average expression value, and the size is expressed as a percentage. B UMAP plots of subclusters of macrophages (left) and proportions of the subgroups in the I-PpoI and WT mice (middle left). C UMAP plots of subclusters of DCs, monocytes and neutrophils (left) and proportions of the subgroups in the I-PpoI and WT mice (middle left). Expression of selected marker genes for each classification (middle right). The color of the dot is proportional to the average expression value, and the size is expressed as a percentage. Violin plots showing the expression levels of selected genes characterized in the clusters (right). The y-axis shows the log-scale normalized read count. D, E Representative immunohistochemical staining images with an anti-CD11b antibody (D) an anti-CD11c antibody (E) and in the kidney cortex (a), the liver b and VAT c at 16 weeks of age. F Representative FACS plots of the kidney cortex a, VAT b, and peripheral blood mononuclear cells (PBMCs) (c) of I-PpoI and WT mice at 16 weeks of age (left). Proportions of CD11b⁺ or CD11c⁺ macro`phages in the kidney and VAT and those of CD11b⁺ Ly6c^hi monocytes in PBMCs, as assessed by flow cytometry (right). In a–c n = 4 samples in each group. The data are presented as the means ± SEMs. *p < 0.05, **p < 0.01 vs. the control. Scale bars, 20 µm (left) and 100 µm (middle and right) in D and E. The same results were obtained by four different samples in each group. Source data are provided as a Source Data file.