Fig. 7: Macrophage depletion restored metabolic alterations in the I-PpoI mice. | Nature Communications

Fig. 7: Macrophage depletion restored metabolic alterations in the I-PpoI mice.

From: DNA damage in proximal tubules triggers systemic metabolic dysfunction through epigenetically altered macrophages

Fig. 7

A Schematic diagram outlining the experimental design for macrophage depletion. Created in BioRender. https://BioRender.com/442s6k4 (B) Representative FACS plots of the kidney cortex a, VAT b, and PBMCs c of the I-PpoI (Tg) and WT mice at 16 weeks of age with or without clodronate (left). Proportions of CD11b+ and CD11c+ macrophages in kidney and visceral adipose tissue and of CD11b+ Ly6chi monocytes in PBMCs, as assessed by flow cytometry (n = 3, 3). C Time course of body weight. D Serum BUN, Cr and TG levels of the I-PpoI and WT mice at 16 weeks of age with or without clodronate. E (left) ip-GTT and AUC for glucose values 0–120 min post glucose load of the I-PpoI and WT mice at 16 weeks of age with or without clodronate. (right) Fasting serum insulin levels of the I-PpoI and WT mice at 16 weeks of age with or without clodronate. F Real-time RT‒PCR analysis of proinflammatory markers in the liver. G Real-time RT‒PCR analysis of proinflammatory markers in visceral adipose tissue. In C–G, n = 4 samples in each group. The data are presented as the means ± SEMs. *p < 0.05, **p < 0.01 vs. the control; N.S., not significant. Source data are provided as a Source Data file.

Back to article page