Fig. 1: CRISPR screening identifies LRP1 as a crucial host factor for SFTSV infection.

a Schematic representation of the genome-wide CRISPR screening used to identify host factors essential for SFTSV infection (constructed by Figdraw.). MEFs were modified with a CRISPR knockout library, and subjected to two rounds of SFTSV infection. The surviving cells were then collected for sequencing analysis. n = 2, with two biological replicates. b Volcano plot of the CRISPR screening results, with log2-fold change representing gene enrichment on the x-axis and the log10 robust rank aggregation (RRA) p-values on the y-axis. p values were calculated using MAGeCK software based on a two-sided test of the negative binomial distribution in positive selection. The top 8 membrane-related proteins, identified by the MAGeCK score, are labeled. c The top 8 membrane-related proteins from the genome-wide screening, ranked by beta score. d Percentage of cells infected with SFTSV (MOI of 1, 24 hpi) in the indicated knockout (KO) cell pools, as measured by immunostaining with a mouse anti-NP antibody. Relative infection levels were normalized to those of the nontargeting (NT) control group. Data are mean ± SD, n = 3 independent experiments. Intracellular viral RNA and protein levels in WT and LRP1-KD MEFs pretreated with siLrp1-1 (100 nM final concentration) or scrambled siRNA (100 nM final concentration) cells. RNA levels were measured by RT‒qPCR (e), and protein levels were assessed by Western blotting (f). Data are mean ± SD, n = 6 independent experiments. Two-tailed unpaired t test, ****P < 0.0001. g Viral titers in the supernatants of WT and LRP1-KD MEFs after infection with SFTSV (MOI of 1) were monitored over time with TCID₅₀ assays. Data are mean ± SD, n = 3 independent experiments. Two-tailed Student’s t-test was used for comparisons between two groups. Source data are provided as a Source Data file.