Fig. 2: LRP1 is required for SFTSV infection.

a LRP1 was knocked out by simultaneously transducing two sgRNAs into MEFs, and monoclonal LRP1 KO cells (K2, K3, K5, K7, and K8) were isolated. Monoclonal cells were infected with SFTSV (MOI of 1, 48 hpi), and the level of the intracellular viral protein NS was assessed by western blotting. n = 2 independent experiments. Intracellular viral RNA and protein levels in WT and LRP1-KO MEFs. RNA levels were measured by RT‒qPCR (b), and protein levels were assessed by Western blotting (c). Data are mean ± SD, n = 6 independent experiments. Two-tailed unpaired t test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns not significant (24 h: WT vs. K3, P = 0.0003; WT vs. K7, P < 0.0001; 48 h: WT vs. K3, P < 0.0001; WT vs. K7, P < 0.0001; 72 h: WT vs. K3, P < 0.0001; WT vs. K7, P < 0.0001). d SFTSV infection in WT and LRP1 KO cells (MOI of 1, 48 hpi) was detected by immunostaining. Nuclei were stained with DAPI, and the virus was stained with mouse anti-SFTSV NP. Scale bars, 200 μm. e Viral titers in the supernatant of WT and LRP1 KO MEF cells after infection with SFTSV (MOI of 1) were monitored over time with TCID₅₀ assays. Data are mean ± SD, n = 3 independent experiments. Two-tailed Student’s t-test was used for comparisons between two groups. Source data are provided as a Source Data file.