Fig. 6: Experimental workflow and results for combination treatment with MEKi and JNKi or PI3Ki. | Nature Communications

Fig. 6: Experimental workflow and results for combination treatment with MEKi and JNKi or PI3Ki.

From: Spike-in enhanced phosphoproteomics uncovers synergistic signaling responses to MEK inhibition in colon cancer cells

Fig. 6

A Experimental workflow depicting inhibitor treatment of cell lines prior to measurement of cell growth and doubling time with live-cell imaging. B Cell doubling time fold changes relative to within replicate controls, 48 h post-treatment with inhibitors. Insets depict doubling times at selected concentrations. C Growth curves of cells within selected treatment conditions, normalized to treatment-start. D, E Similar to panels B, C, these graphs display the effects of combining MEKi with JNKi on cell doubling time and growth curves. Data are presented as mean + standard deviation, shaded ribbons also indicate standard deviation. n ≥ 3. Source data are provided as a Source Data file.

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